We have located links that may give you full text access.
Journal Article
Research Support, Non-U.S. Gov't
Changes in the preserved heart that limit the length of preservation.
BACKGROUND: The variability in donor heart viability following preservation is well known and very dependent on the animal model used and the methods of preservation. Using hypothermia (2 to 4 degrees C) with the University of Wisconsin solution and microperfusion (3 microl/g/min) myocardial functional recovery after reperfusion is 50% to 60% after 18 hours of preservation. Many investigators have found the 18-hour preservation barrier for functional recovery of the heart with different experimental protocols. In previous studies, we have found in normothermic global ischemia that with necrosis there was a significant amount of apoptosis. It was therefore the purpose of this study to investigate the importance of apoptosis in heart preservation and determine if this cellular disruption plays a part in limiting heart viability during recovery from preservation.
METHODS: Using a heterotopic heart transplant model, myocardial function (end-systolic elastance), high energy phosphate changes, metabolic substrate utilization, electron microscopy to determine degree of injury, and histologic changes that included apoptosis and lamin B(1) analyses were conducted on canine hearts preserved for 18 hours and reperfused for 6 hours. The changes were compared with a control group with no preservation.
RESULTS: The 18-hour preserved heart regained approximately 50% to 60% of its original function, with significant decreases in adenosine triphosphate, no irreversible cellular changes (necrosis), and a 6% to 8% increase in apoptotic myocytes with a concomitant 8% decrease in lamin B(1).
CONCLUSIONS: Preserved hearts can be maintained for approximately 12 hours with functional recovery near normal. However, approaching 18 hours, there is an attenuated functional response which may be related to the to development of apoptosis.
METHODS: Using a heterotopic heart transplant model, myocardial function (end-systolic elastance), high energy phosphate changes, metabolic substrate utilization, electron microscopy to determine degree of injury, and histologic changes that included apoptosis and lamin B(1) analyses were conducted on canine hearts preserved for 18 hours and reperfused for 6 hours. The changes were compared with a control group with no preservation.
RESULTS: The 18-hour preserved heart regained approximately 50% to 60% of its original function, with significant decreases in adenosine triphosphate, no irreversible cellular changes (necrosis), and a 6% to 8% increase in apoptotic myocytes with a concomitant 8% decrease in lamin B(1).
CONCLUSIONS: Preserved hearts can be maintained for approximately 12 hours with functional recovery near normal. However, approaching 18 hours, there is an attenuated functional response which may be related to the to development of apoptosis.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
A Guide to the Use of Vasopressors and Inotropes for Patients in Shock.Journal of Intensive Care Medicine 2024 April 14
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app