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Detection of human metapneumovirus from children with acute otitis media.

Akira Suzuki, Oshi Watanabe, Michiko Okamoto, Hiroko Endo, Hisakazu Yano, Mitsuko Suetake, Hidekazu Nishimura
Pediatric Infectious Disease Journal 2005 July
Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.

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