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Research Support, N.I.H., Extramural
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Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.

Ketevan Sidamonidze, Marcia K Peck, Michael Perez, Dennis Baumgardner, Gerald Smith, Vishnu Chaturvedi, Sudha Chaturvedi
Journal of Clinical Microbiology 2012 May
The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.

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