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Evaluation of activity and potential for development of antimicrobial resistance to a new tinted 2% chlorhexidine gluconate / 70% isopropyl alcohol film-forming sterile preoperative skin preparation.

OBJECTIVES: Chlorhexidine gluconate (CHG) is routinely used for skin antisepsis before surgery. Its activity may be affected by formulation ingredients and the presence of organic matter such as blood and proteins. This in vitro study was designed to evaluate the antimicrobial activity of a new CHG skin prep containing a film-forming copolymer, and detect its potential for development of resistance and the potential for cross-resistance to antibiotics after CHG exposure.

METHODS: The antimicrobial activity was evaluated in the presence and absence of serum in an in-vitro time kill study. Emergence of resistance to CHG and cross-resistance with antibiotics procedures were performed in vitro using 10 repository isolates (ATCC) from eight species and 8 clinical isolate strains equal to the repository isolate strains (4 isolates, 2 resistant and 2 non-resistant, per species).

RESULTS: A 5 log10 reduction (99.999%) for all organisms was observed using the copolymer formulation. The activity remained unchanged in the presence of serum. The minimum inhibitory concentration (MIC) did not increase for any of the strains evaluated for emergence of resistance. In addition, there was no change in MIC related to cross-resistance observed for any of the organism/antibiotic combinations tested.

CONCLUSIONS: These results suggest that the film-forming copolymer and the tint in the new CHG skin prep do not interfere with the antimicrobial efficacy even in the presence of an organic soil load, and that the formulations tested show no potential for development of resistance or cross-resistance with antibiotics.

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