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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Lack of T-cell receptor gene rearrangements in cells involved in Langerhans cell histiocytosis.
Cancer 1995 March 2
BACKGROUND: Studies using X-chromosome inactivation assays have recently provided evidence in support of a clonal origin of cells affected by Langerhans cell histiocytosis (LCH). A search for more specific clonal markers has led to the investigation for T-cell receptor (TCR) gene rearrangements in cells affected by LCH.
METHODS: Conventional southern blot analysis was used to investigate the possibility of clonal TCR gene rearrangements in tissues affected by LCH wherever possible, otherwise, a polymerase chain reaction (PCR)-based technique was employed for amplification of rearranged joint (J) and variable (V) segments of the TCR-gamma gene including the N-region. 32P-labeled PCR products were then resolved using nondenaturing polyacrylamide gel electrophoresis.
RESULTS: The results using the PCR-based technique showed a lack of clonal rearrangement of the TCR-gamma gene in affected tissues of eight patients with different stages of LCH. Southern blot analyses performed on two of these samples confirmed germline configurations at both the TCR-C-beta and delta-2 gene loci.
CONCLUSIONS: There is no evidence of clonal TCR gene rearrangement in cells involved by LCH. The search for a more specific clonal marker to address whether "'LCH cells" represent a neoplastic clonal transformation of cells with differentiation toward Langerhans cell phenotype continues.
METHODS: Conventional southern blot analysis was used to investigate the possibility of clonal TCR gene rearrangements in tissues affected by LCH wherever possible, otherwise, a polymerase chain reaction (PCR)-based technique was employed for amplification of rearranged joint (J) and variable (V) segments of the TCR-gamma gene including the N-region. 32P-labeled PCR products were then resolved using nondenaturing polyacrylamide gel electrophoresis.
RESULTS: The results using the PCR-based technique showed a lack of clonal rearrangement of the TCR-gamma gene in affected tissues of eight patients with different stages of LCH. Southern blot analyses performed on two of these samples confirmed germline configurations at both the TCR-C-beta and delta-2 gene loci.
CONCLUSIONS: There is no evidence of clonal TCR gene rearrangement in cells involved by LCH. The search for a more specific clonal marker to address whether "'LCH cells" represent a neoplastic clonal transformation of cells with differentiation toward Langerhans cell phenotype continues.
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